Kit d’ extraction d’ ADN viral Lab-Aid 824s : pour 48 tests
Kit d’ extraction d’ADN viral Lab-Aid, certifié CE.
Disponible sur commande
The Lab Aid 824 DNA extraction system is intended for in vitro diagnostic use. The kit provides high quality DNA that is suitable for direct use in downstream applications, such as amplification or other enzymatic reactions. The Lab Aid 824 DNA extraction system performs all steps of the sample preparation procedure for up to 24 samples in a single run.
The DNA Extraction Kit utilizes magnetic beads technology for automated extraction and purification of human DNA from whole blood, saliva, tissue, blood spot, amniotic fluid, bacteria, blood clot, etc.
Magnetic beads technology combines the speed and efficiency of silica based nucleic acid purification with the convenient handling of magnetic beads. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples. The purification procedure comprises 4 steps: lysis, binding, washing, and elution.
- Lysis: The samples are lysed first in the presence of lysis buffer, which allows the nucleic acids to be released.
- Binding: Binding buffer is added to the lysed samples to adjust binding conditions. Lysates are thoroughly mixed with magnetic beads to allow optimal adsorption of DNA to the silica surface. Salt and pH conditions ensure that protein and other contaminants, which can inhibit PCR and other downstream enzymatic reactions, are not bound to the magnetic beads.
- Washing: While viral nucleic acids and bacterial DNA remain bound to the magnetic beads, contaminants are efficiently washed away during a sequence of wash steps.
- Elution: Highly pure DNA is eluted in elution buffer. The purified nucleic acids can be either used immediately in downstream applications or stored for future use.